年产1000吨淀粉酶生产工艺设计

丁璟剑 方开青 马劲 陈澍泽 蔡锟

（武汉纺织大学 环境工程学院 生物091）

摘要：α-淀粉酶广泛分布于动物、植物和微生物中，能水解淀粉产生糊精、麦芽糖、低聚糖和葡萄糖等，是工业生产中应用最为广泛的酶制剂之一。目前，α-淀粉酶已广泛应用于变性淀粉及淀粉糖、焙烤工业、啤酒酿造、酒精工业、发酵以及纺织等许多行业。本次设计的淀粉酶发酵厂，分别以玉米粉为碳源，以豆饼为氮源，以BF-7658枯草芽孢杆菌为生产菌种，采用深层发酵法，提取工艺采用盐析法，年产1000吨淀粉酶，日产4吨。同时做出了生产工艺流程图，进行了物料衡算和热量衡算，设计了发酵罐和种子罐的尺寸和车间的布置和结构。

关键词：α-淀粉酶；生产工艺设计；深层发酵法

Abstract: alpha amylase widely distributed in animals, plants and microbes, hydrolysis can produce dextrin, maltose starch, oligosaccharides and glucose and so on, it is the most widely used in industrial production of one of the enzyme preparation. At present, the alpha amylase has been widely used in modified starch and starch sugar, baking industry, beer brewing, alcohol industry, fermentation and textile and the many industries. The design of the fermentation plant amylase, respectively, with corn flour for carbon sources, to soybean cake as nitrogen source, with BF-7658 Bacillus subtilis strains for production, the deep fermentation, by salting-out extraction technology method, with the annual production capacity of 1000 tons of amylase, four tons per day. At the same time make the production process flow diagram, the material balance calculations and heat balance calculations, the design and the size of the cans of fermentation tank seeds and workshop layout and structure.

Keywords: alpha amylase; Production process design; Deep fermentation