Microbiology China

微生物学通报

Aug. 20, 2013, 40(8): 1468 1477 © 2013 by Institute of Microbiology, CAS

tongbao@im.ac.cn

摘 要: DNA条形码(DNA barcoding)技术作为一门新兴的物种鉴定方法以其灵敏、精确、方便和客观的优势, 在动植物和微生物的分类鉴定中已经得到广泛应用。真菌鉴定中常用作标准条形码的是核核糖体DNA内转录间隔区(Internal transcribed spacer, ITS), 如今也有一些新型条形码被发现和应用到实际操作中, 如微条形码、ND6、EF3。本文对DNA条形码技术的产生和发展做出了总结, 通过研究其在真菌中应用的实际案例分析了DNA条形码技术的优缺点及发展趋势, 并指出DNA条形码技术将以全新的视角来弥补传统分类学的不足, 最终实现生物自身的序列变异信息与现有形态分类学的结合。 关键词: 真菌, DNA条形码技术, COⅠ, ITS

Advances on DNA barcoding in fungi

ZHOU Jun-Liang ZHAO Rui-Lin*

(Key Laboratory of Forest Disaster Warning and Control in Yunnan Province, Key Subject of Forestry

Protection in Yunnan Province, College of Forestry, Southwest Forestry University,

Kunming, Yunnan 650224, China)

Abstract: As an emerging organism identification method, DNA barcoding has been widely used in plants, animals and microorganisms for its advantage of higher sensitivity, accuracy, and objectivity. Even the nuclear ribosomal internal transcribed spacer (ITS) is used as a standard barcode in fungal identification frequently, nowadays, there are more and more new

基金项目：国家自然科学基金青年项目(No. 31000013); 中科院“西部之光”人才培养计划项目; 云南省中青年科学

技术带头人后备人才培养项目

*通讯作者：Tel: 86-871-3863145; : zhaoruilin@gmail.com 收稿日期：2012-10-17; 接受日期：2012-12-10