应用全二维气相色谱飞行时间质谱分析烟草中性化学成分 鹿洪亮 1,赵明月 2,刘惠民 2,吴正举 1,何阳明 1,梁俐俐 1 (1．福建中烟工业公司技术中心，福建省厦门市海沧区新阳工业区新阳路 1 号 361022； 2．中国烟草总公司郑州烟草研究院，郑州市高新技术产业开发区枫杨街 2 号 450001） 摘要：首次应用全二维气相色谱飞行时间质谱(GC×GC-TOFMS)研究了烟叶的中性化学成分，考察了色谱柱系统、操作条 件（调制周期、柱头压力和初始温度等）对两维分离的影响，建立了烟草挥发性、半挥发性中性化学成分分析的 GC×GC-TOFMS 方法。以云南楚雄产云烟 85C3F 烟叶为例，进行了 GC×GC-TOFMS 与 GC-MS 在定性方面比较，结果显示： 采 用 GC-MS 方法共鉴定出 73 种成分，而 GC×GC-TOFMS 方法共鉴定出 569 种成分，定性结果更加可靠。同时比较了它们的 峰容量、分离能力和灵敏度，结果显示：GC×GC-TOFMS 的峰容量是 GC-MS 的 22 倍，灵敏度提高了 10-82 倍；在 1DGC-MS 上峰形很好的一个峰，被 GC×GC-TOFMS 分离出 6 种成分。还以 C1、C2、C3、C4 取代萘为例对 GC×GC-TOFMS 在族分离方 面的能力进行了研究。 关键词：全二维气相色谱飞行时间质谱、烟草、中性化学成分、同时蒸馏萃取 Analysis of Semi-Volatile Neutral Constituents in Tobacco by GC×GC-TOFMS Lu Hong-Liang(1), Zhao Ming-Yue(2), Liu Hui-Min(2), Wu Zhengju(1), He Yang-ming(1), Liang Li-Li(1) 1. R&D Center of Fujian Tobacco Industrial Corporation, Fujian,361022, China 2. Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, China Abstract: GC×GC-TOFMS was used to analyze tobacco for the first time. The operational conditions of GC×GC-TOFMS were studied, column sets, modulation frequency, column pressure and initial temperature were optimized. Yun85C3F, a flue-cured tobacco, was analyzed in detail. The results showed that GC×GC-TOFMS was more powerful than GC-MS in qualification and quantification studies. 73 constituents were identified in the neutral fraction by GC-MS, whereas 569 constituents were identified by GC×GC-TOFMS. GC×GC-TOFMS was more accurate than GC-MS in identifying unknown compounds; a peak in 1DGC spectrum was isolated into 6 peaks in GC×GC-TOFMS spectrum in the experiment. The modulated sensitivities identified by GC×GC-TOFMS were 16-82 times higher than those identified by GC-TOFMS. The capacity of group classification was also discussed in analyzing C1-C4 alkyl-substituted naphthalene. So the capacity of separation and identification by GC×GC-TOFMS was stronger than GC-MS, GC×GC-TOFMS was suitable to analyze complex matrix. Keywords:

comprehensive two-dimensional gas chromatography ； time-of-flight mass spectrometry ； tobacco ； neutral constituents；GC×GC/TOFMS；SDE 619